I happen to have a bag in front of my keyboard full off all the brass out of a set of 550 carbs. So I thought I'd try this and see what the result was.
Completely different. None of the needles went down fully, in fact they stopped well before the grooves, plus even in that position they were really protruding past the end of the emulsion tubes. It seems the 500/550 needles are considerably longer than the 400s. You'd expect they'd be similar, same style of carb so why are the 400 needles so short? How do they interact with the main jet when they don't protrude past the end of the tube. Have you checked the number stamped on them to see if they are standard? maybe someone swapped them in the past out of a different Honda.